RCF vs. RPM: The One Mistake That Ruins Your Spin

2026-06-15

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Most lab technicians set centrifuges by RPM. But RPM is meaningless without rotor radius.

The real metric? Relative Centrifugal Force (RCF or g-force) – the actual force applied to your sample.

🔁 The simple rule:
Same RPM + smaller rotor = lower g-force = incomplete pelleting
Same RPM + larger rotor = higher g-force = potential cell damage

📐 Quick formula:
RCF = 1.118 * 10⁻⁵ * r * (RPM)²
(*r* = rotor radius in cm)

✅ Best practice: Always validate protocols by RCF, not RPM. When replicating a published method, match the g-force – not the speed.

Many failed experiments trace back to this single oversight. Don't let yours be one of them.

#LabSkills #Centrifugation #RCFvsRPM #ScienceBasics

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